The Role
Develops and optimizes antibody purification, bioconjugation, latex and nanoparticle diagnostic reagents, and immunoturbidimetric assays. Responsibilities include EDC/NHS conjugation, assay validation, DOE studies, troubleshooting, stability evaluation, technical documentation, mentoring, technology transfer, and manufacturing scale-up support. Collaborates with antibody R&D, QC, QA, manufacturing, and multidisciplinary product development teams under GLP/GMP requirements.
Summary Generated by Built In
Yashraj Biotech is seeking a highly motivated Scientist with 4–5 years of industrial experience in antibody bioconjugation, immunoassay development, and diagnostic reagent development. The candidate will work in the development and optimization of antibody-latex conjugates for immunoturbidimetric assays while collaborating with multidisciplinary R&D and Quality teams to deliver robust diagnostic products.
Key Responsibilities
- Design, execute, and optimize antibody purification, characterization, and bioconjugation workflows.
- Develop EDC/NHS-based covalent conjugation strategies for latex and nanoparticle-based diagnostic reagents.
- Optimize antibody loading, blocking conditions, coupling chemistry, and conjugate stability.
- Develop and optimize immunoturbidimetric assays (ITA)
- Design and execute DOE-based optimization studies for conjugation efficiency and assay performance.
- Evaluate analytical performance including sensitivity, specificity, linearity, precision, dynamic range, and robustness.
- Troubleshoot conjugation, assay performance, and reagent stability issues.
- Maintain accurate experimental documentation, SOPs, technical reports, and development records in compliance with GLP.
- Mentor junior scientists, trainees, and interns in laboratory techniques and experimental planning.
- Participate in technology transfer activities and support manufacturing scale-up of developed reagents.
Cross-Functional Responsibilities
- Collaborate with Polyclonal and Monoclonal Antibody R&D teams for antibody selection and characterization.
- Work closely with Quality Control (QC) during analytical validation and product performance evaluation.
- Coordinate with Quality Assurance (QA) to ensure compliance with documentation, change control, and regulatory requirements.
- Support Manufacturing in process optimization and troubleshooting during scale-up.
- Participate in product development meetings, technical reviews, and root-cause investigations.
- Contribute to continuous process improvement and new technology implementation.
Required Technical Skills
Antibody & Protein Sciences
- Strong understanding of monoclonal, polyclonal, artificial polyclonal antibodies.
- Knowledge of antibody structure, affinity, avidity, stability, and antigen-antibody interaction kinetics.
- Experience handling native and recombinant antigens and antibodies.
- Hands-on experience in antibody purification using ammonium sulphate precipitation, dialysis, ultrafiltration, and related techniques.
- Understanding of protein stability and conjugate characterization.
Bioconjugation
- Hands-on industrial experience in EDC/NHS chemistry, Maleimide–Thiol Chemistry, Glutaraldehyde Coupling
- Expertise in antibody immobilization onto latex beads , nanoparticles
- Understanding of surface chemistry, functional group activation, antibody orientation, and conjugation efficiency
- Experience in optimization of conjugation parameters for improved assay performance
Latex/Nanoparticle Diagnostics
- Experience developing latex-based diagnostic reagents.
- Understanding of particle size selection, surface modification, colloidal stability, blocking strategies, and storage stability.
- Knowledge of reagent formulation and long-term stability assessment.
Immunoassays
- Hands-on experience with Immunoturbidimetric Assays (ITA)
- Working knowledge of ELISA and CLIA platforms
- Experience in assay optimization, calibration curve generation, cut-off determination, and analytical validation
Analytical Techniques
Experience with:
- Immunoturbidometry
- UV-Visible Spectrophotometry
- Downstream processing of polyclonal antibodies
- Dynamic Light Scattering (DLS)
- Zeta potential analyser
- Dialysis and ultrafiltration
- Statistical analysis of assay performance
Laboratory Practices
- Excellent laboratory skills including precision pipetting and sample handling.
- Experience working under GLP/GMP environments.
- Knowledge of laboratory safety, contamination control, and documentation practices.
Desired Competencies
- Strong experimental design and scientific problem-solving skills.
- Excellent troubleshooting and analytical thinking.
- Ability to independently manage multiple R&D projects.
- Strong written and verbal scientific communication.
- Experience mentoring junior team members.
- Good organizational and project management skills.
- Ability to work effectively in cross-functional teams.
Skills Required
- 4–5 years of industrial experience in antibody bioconjugation, immunoassay development, and diagnostic reagent development
- Strong understanding of monoclonal, polyclonal, and artificial polyclonal antibodies
- Knowledge of antibody structure, affinity, avidity, stability, and antigen-antibody interaction kinetics
- Experience handling native and recombinant antigens and antibodies
- Hands-on antibody purification experience, including ammonium sulfate precipitation, dialysis, and ultrafiltration
- Hands-on experience with EDC/NHS, Maleimide-Thiol, and Glutaraldehyde coupling chemistries
- Expertise immobilizing antibodies onto latex beads and nanoparticles
- Experience developing latex-based diagnostic reagents
- Hands-on experience with immunoturbidimetric assays
- Working knowledge of ELISA and CLIA platforms
- Experience with assay optimization, calibration curves, cut-off determination, and analytical validation
- Experience with immunoturbidometry, UV-Visible spectrophotometry, Dynamic Light Scattering, and zeta potential analysis
- Experience working under GLP/GMP environments
- Strong experimental design, troubleshooting, analytical thinking, and scientific communication skills
- Ability to independently manage multiple R&D projects and work cross-functionally
- Experience mentoring junior team members
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The Company
What We Do
Yashraj Biotechnology Limited develops and manufactures high-purity native and recombinant protein biomarkers for diagnostic applications. Established in 1999, the company also addresses preclinical drug-discovery needs and operates as an emerging contract research organization specializing in preclinical research. Its biotechnology solutions support diagnostic development and related life-science workflows, with operations headquartered in Mumbai and offices in the United States and Germany for international customers.
