Biochemist I - Recombinant Protein Manufacturing

Reposted Yesterday
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San Diego, CA
In-Office
23-27 Hourly
Entry level
Biotech
The Role
Responsible for producing recombinant protein products, including protein expression, purification, quality support, and maintaining production records and inventory.
Summary Generated by Built In
Job TitleBiochemist I - Recombinant Protein Manufacturing
Location(s)
San Diego - BioLegend

About Us

Revvity is a developer and provider of end-to-end solutions designed to help scientists, researchers, and clinicians solve the world’s greatest health challenges. We pair the enthusiasm of an industry disruptor with the experience of a longtime leader. Our team of 11,000+ colleagues from around the globe are vital to our success and the reason we’re able to push boundaries in pursuit of better human health.

Find your future at Revvity 

Job Summary

BioLegend is seeking a Biochemist I join our recombinant protein manufacturing team. The Biochemist I is responsible for producing recombinant protein products using bacterial cell culture systems. This role involves multiple stages of production, including protein expression, purification, and quality support. Key responsibilities include preparing buffer solutions, culture media, and dilution reagents; performing bacterial cell cultures; purifying recombinant proteins via affinity methods; and conducting DNA isolations following standard operating procedures.

Additional duties may involve transferring products for downstream testing, maintaining production records and inventory, and preparing documentation related to products and projects.

The ideal candidate is an organized, collaborative team player with strong verbal and written communication skills and a solid understanding of recombinant protein manufacturing methodologies.

Essential Functions

Reasonable accommodation may be made to enable individuals with disabilities to perform the essential functions.

  • Perform DNA isolation, prepare various buffers and reagents for experiment of recombinant protein projects.
  • Perform transformation, culture and harvest culture of bacterial cells.
  • Clarify and process the culture supernatant/cell pellet/inclusion body to capture, extract, or denature/refold recombinant proteins.
  • Purify of recombinant proteins & antibodies by affinity chromatography.
  • Monitor protein processing by SDS-PAGE for various protein production stages.
  • Perform protein bulk processing (concentration, dilution, buffer change), purity analysis and endotoxin removal
  • Support inventory management of raw material and plasmid constructions for protein production
  • Assist in transferring products across different departments for QC or other inventory-related activities.
  • Maintain batch records and other documentations that are essential to be in compliance with ISO13485:2016
  • Support the preparation of material and checking for documents or historical records are available for incoming tissue and insect culture work orders.
  • Support bottling of recombinant protein products
  • Maintain general upkeep of the lab.
  • Other projects or responsibilities may be required.

Minimum Qualifications – Education and Experience

  • Bachelor of Science in in Life Science or related area with 6 months of working experience in related biotech manufacturing environment.

Preferred Qualifications – Education and Experience

  • Bachelor of Science in in Life Science or related area with at least 12 months of working experience in related biotech manufacturing environment.
  • Experience in DNA isolation and bacterial culture.
  • Experienced in purification and formulation of recombinant protein.

Applicants must be currently authorized to work in the United States on a full-time basis for any employer.

Work Environment & Physical Demands

Lab

While performing the duties of this job, the employee regularly works in a laboratory environment with occasional data entry on a computer. Occasional exposure to a warehouse environment.

The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job.

While performing the duties of this job, the employee is regularly required to use hands and fingers to handle, feel, or operate objects, tools or controls, and reach with hands and arms. The employee is frequently required to stand, talk and hear.

The base salary range for this full-time position is $22.80 - $27.00/hr. This range reflects the minimum and maximum target for a new hire in this position. The base pay actually offered to the successful candidate will take into account internal equity, work location, and additional factors, including job-related skills, experience, and relevant education or training. Your recruiter can share more about the specific salary range for your preferred location during the hiring process. 

Please note that base pay is only one part of our total compensation package and is determined within a range. This range allows for the successful candidate to have an opportunity to progress within the position and develop at our company. This base pay range does not take into account bonuses, equity, or other benefits which may be applicable and are dependent on the level and position offered.

What do we offer?

We provide competitive and comprehensive benefits to our employees. Below are some highlights of our benefits:

  • Medical, Dental, and Vision Insurance Options

  • Life and Disability Insurance  

  • Paid Time-Off

  • Parental Benefits

  • Compassionate Care Leave

  • 401k with Company Match

  • Employee Stock Purchase Plan

Learn more about Revvity’s benefits by visiting our Bswift page. Log-In instructions are provided towards the bottom of the Bswift page.

*For benefit-eligible roles only. Part-time and temporary roles may not be eligible for all benefits listed. Please reach out to your recruiter for more information.

Revvity is an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to any characteristic or status protected by applicable federal, state, and/or local laws. If you are an applicant with a disability that requires reasonable accommodation to complete any part of the application process or are limited in the ability—or unable to use—the online application system and need an alternative method for applying, you may contact [email protected].

Top Skills

Affinity Chromatography
Bacterial Cell Culture
Iso13485:2016
Protein Expression
Purification
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The Company
Waltham, , Massachusetts
3,109 Employees
Year Founded: 2023

What We Do

At Revvity, “impossible” is inspiration, and ""can't be done"" is a call to action. Revvity provides health science solutions, technologies, expertise and services that deliver complete workflows from discovery to development, and diagnosis to cure. Revvity is revolutionizing what’s possible in healthcare, with specialized focus areas in translational multi-omics technologies, biomarker identification, imaging, prediction, screening, detection and diagnosis, informatics and more. With more than $3 billion in revenue and over 11,000 employees, Revvity serves customers across pharmaceutical and biotech, diagnostic labs, academia and governments. It is part of the S&P 500 index and has customers in more than 190 countries. Learn more at www.revvity.com

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