with a strong background in biochemical assays to join our dynamic team and contribute to building our pipeline for kidney diseases. The successful candidate will play a crucial role in supporting our research efforts working alongside a team of scientists. The successful candidate will lead the execution of high-resolution protein and nucleic acid analyses,
bridging in vitro mammalian cell biology and in vivo tissue studies. We are looking for a versatile team member with a rigorous approach to data quality, a background in multi-modal assay development (including protein and RNA workflows), and a commitment to maintaining a safe, collaborative laboratory environment.
Responsibilities
- Execute routine and complex Western blots to assess target protein expression levels across diverse biological matrices.
- Develop and optimize protein purification, labeling and enrichment protocols to isolate protein targets and study molecular interactions
- Perform RNA-based assays (such as RNA extraction and RT-PCR) to validate target modulation and cellular responses.
- Adapt and execute assays in high-throughput formats to support validation of compound activity across programs.
- Analyze experimental results using software (such as ImageJ) and Graphpad Prism; maintain meticulous electronic laboratory notebooks (ELN).
- Adhere to strict laboratory safety guidelines, ensure a clean and organized work environment, and contribute to development of robust, reproducible SOPs
- Collaborate within the Discovery Biology team to optimize assay conditions, troubleshoot technical hurdles, interpret outcomes, and ensure consistent assay reproducibility
- Communicate findings to support aggressive project timelines and deliverables
Qualifications
- Bachelor's or Master’s degree in a relevant scientific field with at least 3-5 years of hands-on experience in protein biochemistry techniques. Prior biotechnology industry experience is required.
- Proven proficiency in both protein and nucleic acid techniques. Familiarity with automated protein analysis systems, such as the ProteinSimple Jess (Simple Western) is a plus.
- Demonstrated ability to troubleshoot and refine biochemical protocols with minimal
supervision. - Strong attention to detail with excellent organizational and time management skills.
- Effective communication and interpersonal skills, with the ability to work collaboratively in a team environment.
- Commitment to maintaining high-quality standards and adhering to laboratory safety protocols.
Skills Required
- Bachelor's or Master's degree in a relevant scientific field
- 3-5 years of hands-on experience in protein biochemistry techniques
- Proficiency in protein and nucleic acid techniques
- Ability to troubleshoot and refine biochemical protocols
- Effective communication and interpersonal skills
What We Do
5AM was founded in 2002 and pursues investments in advanced life science innovations, and seeks to be diversified across the biopharmaceutical therapeutics, and platform technologies. Within each sector, 5AM invests across multiple therapeutic areas and modalities and evaluates opportunities based on innovative platform technologies, corporate spin-offs and products with shorter development cycles. Content 5AM shares on this platform is not, and may not be relied on, in any manner, as legal, tax, investment, accounting or other advice or as an offer to sell or a solicitation of an offer to buy an interest in any private fund, special purpose or other investment vehicle sponsored by 5AM Ventures. Content 5AM shares on this platform is not an investment recommendation. For a full list of all 5AM portfolio companies, please visit: https://5amventures.com/portfolio/
